TaqMan Universal Master Mix II has been validated for use with genotyping assays. This extended benchtop stability provides flexibility to process numerous samples using high-throughput liquid handling systems that may require assembled reactions to sit at room temperature until they can be fed into the real-time PCR instrument. TaqMan Universal Master Mix II is formulated with high-quality components that retain the ability to discriminate between small relative differences in target concentration even after incubating assembled PCR reactions for 24 hours at room temperature. TaqMan Universal Master Mix II is formulated to deliver the necessary specificity to distinguish between these highly homologous targets.īenchtop Stability for High-Throughput Handling Some members of microRNA families differ from each other by as little as 1 nucleotide, presenting a difficult challenge for many master mixes. MicroRNAs are small (21–23 nt) nucleic acids that are involved in the regulation of many genes and have been implicated in disease processes such as cancer and heart disease.
The master mix also provides excellent specificity in real-time PCR. Specificity to Distinguish Highly Homologous Targets TaqMan Universal Master Mix II provides dependable target quantification over a wide dynamic range of expression levels, so that you can detect and quantitate high and low expressors in a single experiment. Sensitivity and Wide Dynamic Range for Gene Expression Analysis TaqMan Universal Master Mix II brings the quality and reliability you’ve come to expect from Applied Biosystems to an improved master mix formulation for real-time PCR.